Eurizon.eu

Organization: University of Greifswald, Institute of Pharmacy, Department of Pharmaceutical Biotechnology

Location: Greifswald, Germany

Field: Biology

Requirements:

Applicants should have training and research experience in microbiology, molecular biology, marine biology, bioinformatics, or related disciplines. Very good knowledge of English in writing and speaking is required. Applicants must satisfy the eligibility criteria for Marie Curie Initial Training Networks. Students should have an MSc or equivalent degree that enables them to begin their PhD studies.

Abstract:

Symbioses between chemosynthetic bacteria and marine invertebrates occur in a variety of environments, such as deep sea hydrothermal vents or cold seeps.

Description:

To obtain a mechanistic understanding of these symbioses the following techniques will be applied: i) Density gradient centrifugation techniques will be used to separate host and symbiont cells and to separate the specific symbiont cell fractions of the dual mussel symbiosis. ii) Symbiont protein extracts will be separated by gel-based (1D and 2D PAGE) and gel-free (HPLC) methods. MS/MS analyses will be applied to determine the cytoplasmic and membrane proteomes of the bacteria. The identification of symbiont proteins, as a prerequisite for the detailed reconstruction of the major metabolic pathways, requires access to the respective symbionts’ genome sequences. While the Riftia symbiont metagenome is publically available, iii) metagenome analyses of the Bathymodiolus symbionts need to be performed in collaboration with the MPI in Bremen. For the evaluation of the obtained identification results from 2D gels, the Delta2D software will be used to detect, quantify and compare symbiont-specific proteomic signatures. iv) Electron and confocal microscopy will be applied to determine the structural distribution of the bacteria inside the host (in collaboration with the University of Vienna). v) Finally, Nano-SIMS analyses of the elemental and isotopic composition of the bacteriocytes and host tissue are planned.
 
 

Deadline: 01-07-2011

Contacts:

Link: http://pharm1.pharmazie.uni-greifswald.de/biot/en/index.html

Email: schweder@uni-greifswald.de

Phone number: 0049 3834 864212

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