Eurizon.eu

Organization: University of Copenhagen

Location: Copenhagen, Denmark

Field: Synthetic Biology

Requirements:

A MSc or equivalent degree in a field relevant to the research project and good English skills are required

Abstract:

Fully funded PhD positions are available in the area of Synthetic Biology as of 1st November 2011 or soon thereafter. The successful PhD fellows will be part of the UNIK research program in Synthetic Biology at the University of Copenhagen

Description:

The following PhD position is available.
Detection of protein dynamics using single molecule fluorescence confocal microscopy.
 
The ability to detect the fluorescence of a single fluorophore has led to great advances in understanding of heterogeneity and dynamics at the nanoscale level in life and material sciences. The specific emission properties of fluorophores like its intensity, decay time, emission wavelength (spectrum) and anisotropy can be used to probe changes in the fluorophores surrounding. For example, fluorescence decay time changes can be used as a powerful tool to monitor quenching and energy transfer processes. A confocal microscope setup will be developed with multiple avalanche photodiodes and a spectrometer (polychromator/CCD camera) that can be used to monitor changes in multiple parameters of the single fluorophore´s emission at the same time, providing a wealth of information. In order to extract as much information as possible, it is important to choose fluorophores with a high absorption coefficient, high fluorescence, low triplet, and low photobleaching quantum yield. For this, organic fluorophores (with and without the presence of photostability enhancing additives, like e.g. ROXS) will be tested and compared with a new class of silver cluster based emitters, attached to proteins in order to determine their photo stability performance. As a test case we will investigate the conformational changes that occur during binding and transport of substrate within the neurotransmitter transporter homologue, LeuT
 
Salary and employment
Salary is paid according to the agreement between the Ministry of Finance and the Danish Federation of Professional Associations (AC). PhD employment is for a total period of 3 years.
 
Application
Written application marked "UNIK PhD stipend" and the relevant project title should be received no later than the 1st of April 2011, 12 noon. The application should contain a short description of own research interests, motivation letter, a CV including publication list, copy of MSc Diploma, or equivalent and supplementary information of relevance for the position, together with name and address of references.
 
Inquiries regarding the project can be sent to tom@kemi.ku.dk.
 
The University of Copenhagen wishes to reflect the surrounding society and encourage all qualified applicants, regardless of personal background, to apply for the positions.
 
Principal supervisor
Associate professor
Tom Vosch
Direct Phone: (+45) 3532 0313
E-mail: tom@kemi.ku.dk
Department of Chemistry / Nano-Science Center, KU-Science
 
Co-supervisor
Professor
Ulrik Gether
Direct Phone: (+45) 3532 7602
E-mail: gether@sund.ku.dk
Molecular Neuropharmacology Grp / Dept. of Pharmacology
 
Co-supervisor
Associate professor
Claus Juul Løland
Direct Phone: (+45) 3532 7602
E-mail: gether@sund.ku.dk
Molecular Neuropharmacology Grp / Dept. of Pharmacology
 
 
Please send your application, marked "UNIK PhD-stipend", to:
 
Nanna Heinz, UNIK coordinator
Department of Plant Biology and Biotechnology, KU-LIFE
heinz@life.ku.dk

Deadline: 01-04-2011

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