PhD in development of enhanced photoswitchable fluorescent proteins
Organization: University of Amsterdam
Location: Amsterdam, Netherlands
Field: Physics
Requirements:
A master/academic degree in Biochemistry, Cell biology or Molecular biology (or related fields). The candidate should have experience with molecular biological techniques (cloning, PCR), protein production and characterization techniques. Affinity with microscopy and spectroscopy is recommended. In addition the applicant should have the ability to work in a team, to work independently, to publish and present scientific research in English, to quickly gather and master scientific knowledge & concepts. Strong communication and organizational skills, problem solving skills and perseverance are recommended
Abstract:
Fluorescent proteins (FPs) have revolutionized molecular cell biology and the more recent development of photoswitchable fluorescent proteins (psFPs) holds promise for even further boosts in this dynamic scientific field. In conjunction with parallel development of advanced optical imaging methodologies, these psFPs will allow novel avenues for quantitative (multi-color) single-molecule microscopy in live cells.
Description:
Fluorescent proteins (FPs) have revolutionized molecular cell biology and the more recent development of photoswitchable fluorescent proteins (psFPs) holds promise for even further boosts in this dynamic scientific field. In conjunction with parallel development of advanced optical imaging methodologies, these psFPs will allow novel avenues for quantitative (multi-color) single-molecule microscopy in live cells. The first (currently available) generation psFPs is limited in number and with respect to their photophysical characteristics. In this NWO-Echo project a second generation of psFP with enhanced properties will be engineered. After successful development, the psFPs will be used to label and study the localization, mobility and molecular interactions of proteins involved in G-protein signaling. This project builds on extensive expertise in the group in fluorescent protein optimization and investigation of protein signaling networks, integrating molecular technologies and microscopy. The project will combine (i) protein engineering, production and characterization using molecular cloning and evolution strategies with (ii) microscopic approaches to test and apply the developed psFPs to study G-protein signaling in cultured mammalian cells.
The full-time appointment will be on a temporary basis for a maximum period of four years (18 months plus a further 30 months after a positive evaluation) and should lead to a dissertation (PhD thesis). An educational plan will be drafted that includes attendance of courses and (international) meetings. The PhD student is also expected to assist in teaching of undergraduates. Based on a full-time appointment (38 hours per week) the gross monthly salary will range from € 2042 in the first year and € 2612 in the final year, according to the Dutch salary scales for PhD students. The collective employment agreement (CAO) of Dutch universities is applicable.
Deadline: 30-06-2011
Contacts:
Link: http://www.academictransfer.com/employer/UVA/vacancy/10037/apply/
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